Although overexpression of ABI5 confers hypersensitivity to ABA and sugar, as previously described for ABI4 and ABI3 overexpression lines, it has relatively limited effects on enhancing ABA-responsive gene expression.

osmotic stress. miR172b overexpression and snz-1 exhibited increased sensitivity to ABA and osmotic stress during specific post-germinative stage, and resulted in higher expression of ABI3, ABI5 and downstream genes, such as Em6 and RAB18, than wild type under ABA treatment.

(A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ Moreover, overexpression of ABI5 (ABI5-OE-8) in the JAZ8-ΔJas ABI3-OE background rendered the transgenic lines much more sensitive to ABA and COR during seed germination . Similar data were also obtained when ABI3 and ABI5 were expressed in the JAZ5-ΔJas background ( Supplemental Figures 12C and 12D ). Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions.

Abi5 overexpression

Together, our results reveal a previously uncharacterized signaling module in which JAZ Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas … ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASEexpression and cata- lase activity. Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Bar, 1 cm.

ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001). Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001).

HY5 protein interacts with theABI5 promoter, and thein vivo interaction was enhanced by ABA. Ectopic expression of ABI5 in the hy5 mutant background restored its ABA sensitivity and overexpression of ABI5 in the wild-type background-enhanced light response. These results The relative hypocotyl lengths of Col-0, abi5-1, and abi5/ZFP3ox seedlings were similar at different fluence rates of red light. In contrast to the Col-0 background, ZFP3 overexpression could not reduce relative hypocotyl lengths in the abi5-1 mutant background upon red light illumination .

ZFP3 overexpression in the Col-0, abi2-1, and abi4-. 101 backgrounds reduced hypocotyl lengths by. 50% to 60%, while in abi5-1/ZFP3ox seedlings, hy-.

Moreover, the expression of eight other PYLs is … Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. ABI5 production is enhanced by stress including high salt and drought.

By analogy to the related Novel Interactor of JAZ (NINJA) protein, it was suggested that the AFPs interact with the co-repressor TOPLESS to inhibit ABA-regulated gene expression. 2020-05-08 The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. In sleepy1 mutant seeds, RGL2 overaccumulates; germination of these seeds can occur under conditions that produce low ABI5 … Overexpression of ABI5 in Col-0 influences flowering time To assess the correlation between flowering time and ABI5 expression, overexpressing transgenic lines carrying plas-mid p35S::ABI5-HA in Col-0 background were generated. A total of 43 lines were analysed by examining ABI5-HA protein level in individual transgenic plants (Fig. 1A). In the ABA-ABAR-WRKY40-ABI5 core signaling complex, WRKY40 acts to suppress ABA signaling, and high levels of ABA promote ABAR-WRKY40 interactions, then activate expression of ABA responsive genes, including the ABA responsive transcription factors ABI5 and ABI4. J. Exp. Bot., 2017.
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ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASE expression and catalase activity. Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. ABI5 overexpression rescued the growth arrest phenotype at low CO 2.

Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas exchange when plants experience periodic drought. However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation.
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Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

2020-03-02 · Consistently, PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark. Moreover, we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9, and that this interaction is not regulated by ABA. Overexpression of ABI5 results in the plants being hypersensitive to abscisic acid such that they respond to low levels of abscisic acid which have no effect on wild type plants. One aspect of the invention is seeds, seedlings or plants which are resistant to drought because they overexpress ABI5. An ABI5 overexpression transgene was constructed by fusing an ABI5 cDNA into pGA643 (An et al., 1988) with 23 bp of 5′-UTR between the cauliflower mosaic virus 35S promoter and the initiating codon of ABI5.

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Although overexpression of ABI5 confers hypersensitivity to ABA and sugar, as previously described for ABI4 and ABI3 overexpression lines, it has relatively limited effects on enhancing ABA-responsive gene expression.

In the abi5-7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants. Although overexpression of ABI5 confers hypersensitivity to ABA and sugar, as previously described for ABI4 and ABI3 overexpression lines, it has relatively limited effects on enhancing ABA-responsive gene expression.